Aktivitas insektisida ekstrak Piper aduncum dan Aglaia odorata terhadap Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae)
Insecticidal activity of Piper aduncum and Aglaia odorata extracts on Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae)
DOI:
https://doi.org/10.5994/jei.22.1.52Keywords:
botanical insecticide, Brassicaceae, diamondback moth, feeding inhibition, toxicityAbstract
One of the main problems in the cultivation of Brassicaceae vegetable plants is the attack of diamondback moth Plutella xylostella (Linnaeus). Extracts of Piper aduncum and Aglaia odorata have the potential to be used as botanical insecticides because they contain compounds that are toxic to insects. This study aims to evaluate the toxicity and feeding inhibition activity of hexane-extract of P. aduncum fruits and methanol-extract of A. odorata twigs against second instar larvae of diamondback moth. Extraction was carried out using the maceration method with a powder-to-solvent ratio of 1:10 (w/v). The diamondback moth insects used were from colonies reared in the laboratory. The lethal effect test was conducted using the leaf-dip method with test concentrations equivalent to LC15, LC35, LC55, LC75, LC95, and a control. The feeding inhibition test was conducted using the choice-test method with test concentrations equivalent to LC15, LC35, LC55, and a control. The results show that the hexane-extract of P. aduncum fruit is more toxic to second instar P. xylostella larvae than the methanol-extract of A. odorata twigs based on the probit analysis value of the relationship between concentration and mortality. The LC50 and LC95 values of P. aduncum extract were 0.07% and 0.14%, respectively, while the LC50 and LC95 values of A. odorata extract were 1.63% and 4.72%. Additionally, P. aduncum and A. odorata extracts also exhibited feeding against diamondback moth at concentrations of 0.07% and 1.77%, respectively. The combined effects of direct toxicity and feeding inhibition contributed to the mortality of the test insects. Thus, these two extracts, escpecially P. aduncum extract wich demonstrated higher effectiveness, have a great potential as botanical insecticides for controlling diamondback moth in Indonesia.
Downloads
References
[APRD] Arthropod Pesticide Resistance Database. Arthropod Pesticide Resistance Database, Michigan State University. Arthropod Pestic Resist Database, Michigan State Univ. https://www.pesticideresistance.org/. [accessed 7 July 2023].
Agarwal G, Chang LS, Soejarto DD, Kinghorn AD. 2021. Update on phytochemical and biological studies on rocaglate derivatives from Aglaia species. Planta Medica. 87:937–948. DOI: https://doi.org/10.1055/a-1401-9562.
Anggraini M. 2019. Aktivitas Insektisida Campuran Ekstrak Buah Piper aduncum, Buah Sapindus rarak, dan Daun Tephrosia vogelii terhadap Larva Plutella xylostella (L.) (Lepidoptera: Plutellidae). Tesis. Bogor: Institut Pertanian Bogor.
Anggraini D, Rustam R. 2023. Efektivitas ekstrak daun sirih hutan (Piper aduncum L.) dengan pelarut organik dalam mengendalikan ulat grayak jagung (Spodoptera frugiperda J. E. Smith) di laboratorium. Jurnal Agroteknologi. 13:77–84. DOI: https://doi.org/10.24014/ja.v13i2.17600.
Araújo MJC, Camara CAG, Moraes MM, Born FS. 2020. Insecticidal properties and chemical composition of Piper aduncum L., Lippia sidoides Cham. and Schinus terebinthifolius Raddi essential oils against Plutella xylostella L. Anais de Academia Brasileira de Ciencias. 92:e20180895. DOI https://doi.org/10.1590/0001-3765202020180895.
Basana IR, Prijono D. 1994. Insecticidal activity of Aqueous seed extracts of four species of Annona (Annonaceae) against cabbage head caterpillar, Crocidolomia binotalis Zeller (Lepidoptera: Pyralidae). Buletin Hama dan Penyakit Tumbuhan. 7:50–60.
Batan A, Simanjuntak P, Barong Tongkok J. 2018. Isolasi senyawa kimia aktif antioksidan dari fraksi etil asetat daun sirih hutan (Piper aduncum l.) Isolation of chemical active compounds antioxidant from ethyl acetate fraction of betel leaf forest (Piper aduncum L.). Jurnal Atomik. 3:83–90.
Bernays EA CR. 1994. Host-Plant Selection by Phytophagous Insects. New York: Chapman & Hall. DOI: https://doi.org/10.1007/b102508.
Cossolin JFS, Pereira MJB, Martínez LC, Turchen LM, Fiaz M, Bozdoğan H, Serrão JE. 2019. Cytotoxicity of Piper aduncum (Piperaceae) essential oil in brown stink bug Euschistus heros (Heteroptera: Pentatomidae). Ecotoxicology. 28:763–770. DOI: https://doi.org/10.1007/s10646-019-02072-8.
Carmen ED, Braulio MF, Adriana G, Portero IB, Carmen López-Balboa, Liliana Ruiz-V, Azucena González-Coloma. 2023. Insect antifeedant Benzofurans from Pericallis species. Molecules. 28:975–993. DOI: https://doi.org/10.3390/molecules28030975.
Dadang 2023. Pengembangan Insektisida Nabati untuk Pertanian. Bogor: IPB Press.
Díaz CE, Fraga BM, G Portero A, Brito I, López-Balboa C, Ruiz-Vásquez L, González-Coloma A. 2023. Insect antifeedant benzofurans from Pericallis species. Molecules. 28:975–994. DOI: https://doi.org/10.3390/molecules28030975.
Durofil. 2021. Piper aduncum essential oil: A promising insecticide, acaricide and antiparasitic . A review. 28:42. DOI: https://doi.org/10.1051/parasite/2021040.
Duong NT, Edrada-Ebel R, Ebel R. 2014. New rocaglamide derivatives from Vietnamese Aglaia species. Natural Product Communications. 9:833–834. DOI: https://doi.org/10.1177/1934578X1400900627.
Fazolin M, Monteiro AFM, Bizzo HR, Gama PE, Viana LO, Lima MÉC. 2021. Insecticidal activity of Piper aduncum oil: Variation in dillapiole content and chemical and toxicological stability during storage. Acta Amazonica. 52: 179–188. DOI: https://doi.org/10.1590/1809-4392202102292.
Hasyim DM. 2011. Potensi Buah Sirih Hutan (Piper aduncum) sebagai Insektisida Botani terhadap Larva Crocidolomia pavonana. Tesis. Bogor: Institut Pertanian Bogor.
Janprasert J, Isman B, Wiriyachitra P, Towers G. 1993. Rocaglamide, a natural benzofuran insecticide from Aglaia odorata. Journal of Phytochemistry. 32:67–69. DOI: https://doi.org/10.1016/0031-9422(92)80108-Q.
Kotta NRE, Trisyono YA, Wijonarko A. 2018. Resistance level of Plutella xylostella L. (Lepidoptera: Plutellidae) on Cypermethrin in the Regency of Kupang. Jurnal Perlindungan Tanam Indonesia. 22:186 186–192. DOI: https://doi.org/10.22146/jpti.24750.
Lina EC, Hidayatullah MS, Reflin R, Nelly N. 2023. The activity of spiked pepper fruit essential oil against fall armyworm larvae. IOP Conference Series: Earth and Environmental Science. 1228:012001. DOI: https://doi.org/10.1088/1755-1315/1228/1/012001.
Machado BB, Orue JPM, Arruda MS, Santos CV, Sarath DS, Goncalves WN, Silva GG, Pistori H, Roel AR, Rodrigues-Jr JF. 2016. BioLeaf: A professional mobile application to measure foliar damage caused by insect herbivory. Comput Electron Agric. 129:44–55. DOI: https://doi.org/10.1016/j.compag.2016.09.007.
Magrini FE, Specht A, Gaio J, Girelli CP, Migues I, Heinzen H, Cesio V. 2015. Antifeedant activity and effects of fruits and seeds extracts of Cabralea canjerana canjerana (Vell.) Mart. (Meliaceae) on the immature stages of the fall armyworm Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae). Industrial Crops and Products. 65:150–158. DOI: https://doi.org/10.1016/j.indcrop.2014.11.032.
Mahfud RI. 2016. Formulasi Ekstrak Tanaman Aglaia odorata dan Piper aduncum Untuk Pengendalian Ulat Krop Kubis Crocidolomia pavonana (F.) (Lepidoptera: Crambidae). Tesis. Bogor: Institut Pertanian Bogor.
Mendes JAD, Ratna ES. 2017. Efek mortalitas dan penghambatan makan beberapa ekstrak tumbuhan asal Kabupaten Merauke, Papua terhadap larva Crocidolomia pavonana (F.) (Lepidoptera: Crambidae). Jurnal Hama dan Penyakit Tumbuhan Tropika. 16:107–114. DOI: https://doi.org/10.23960/j.hptt.216107-114.
Mokodompit TA, Koneri R, Siahaan P, Tangapo AM. 2013. Uji ekstrak daun Tithonia diversifolia sebagai penghambat daya makan Nilaparvata lugens Stal. pada Oryza sativa L. Jurnal Bioslogos. 3:50–56. DOI: https://doi.org/10.35799/jbl.3.2.2013.4430.
Morais VP, Cabral FV, Fernandes CC, Miranda MLD. 2023. Brief review on Piper aduncum L., its bioactive metabolites and its potential to develop bioproducts. Brazilian Archives of Biology and Technology. 66:1–11. DOI: https://doi.org/10.1590/1678-4324-2023220314.
Ngo NTN, Ngân D, Le H, Nguyên LT, Trinh B, Nguyen H, Pham D, Dang S, Nguyen Lien-Hoa. 2021. Chemical constituents of Aglaia elaeagnoidea and Aglaia odorata and their cytotoxicity. Natural Product Research. 36:1–9. DOI: https://doi.org/10.1080/14786419.2021.1893723.
Nugroho BW, Edrada RA, Wray V, Witte L, Bringmann G, Gehling M, Proksch P. 1999. An insecticidal rocaglamide derivatives and related compounds from Aglaia odorata (Meliaceae). Phytochemistry. 51:367–376. DOI: https://doi.org/10.1016/S0031-9422(98)00751-1.
Nugroho LH, Pratiwi R, Soesilohadi RCH, Subin ER, Wahyuni S, Hartini YS, Lailaty IQ. 2020. Repellent activity of Piper spp. leaves extracts on rice ear bugs (Leptocorisa oratorius Fabricius) and the characters of its volatile compounds. Annual Research & Review in Biology. 35:34–45. DOI: https://doi.org/10.9734/arrb/2020/v35i930269.
Obermann W, Azri MFD, Konopka L, Schmidt N, Magari F, Sherman J, Silva LMR, Hermosilla C, Ludewig AH, Houhou H, et al. 2023. Broad anti-pathogen potential of DEAD box RNA helicase eIF4A-targeting rocaglates. Scientific Reports. 13:1–15. DOI: https://doi.org/10.1038/s41598-023-35765-6.
Prijono D, Syahputra E, Sudarmo, Nugroho BW, Simanjuntak P. 2006. Aktivitas lima jenis insektisida alami terhadap ulat krop kubis Crocidolomia binotalis Zeller. In: Prosiding Seminar Nasional III. Pengelolaan Serangga yang Bijaksana Menuju Optimasi Produksi. (Bogor, 6 Nov 2001). pp. 72–82. Bogor: Perhimpunan Entomologi Indonesia.
Rohimatun, Aisyah M, Puspasari L, Rusmin D. 2023. Toxicity and chemical compounds of Piper aduncum fruit extract against storage pest Sitophilus oryzae and Callosobruchus maculatus. IOP Conference Series: Earth and Environmental Science. 1253:1–9. DOI: https://doi.org/10.1088/1755-1315/1253/1/012001.
Russianzi W, Prijono D. 2019. Synergistic activity of mixtures of Piper aduncum fruit extract and three microorganism-derived insecticides against the diamond back moth, Plutella xylostella. Cropsaver: Journal of Plant Protection. 2:7–14. DOI: https://doi.org/10.24198/cs.v2i1.22299.
Salehi B, Zakaria ZA, Gyawali R, Ibrahim SA, Rajkovic J, Shinwari ZK, Khan T, Sharifi-Rad J, Ozleyen A, Turkdonmez E, et al. 2019. Piper species: A comprehensive review on their phytochemistry, biological activities and applications. Molecules. 24:1364–1482. DOI: https://doi.org/10.3390/molecules24071364.
Sokolinskaya E, Kolesov D, Lukyanov K, Bogdanov A. 2020. Molecular principles of insect chemoreception. Acta Naturae. 12:81–91. DOI: https://doi.org/10.32607/actanaturae.11038.
Taher M, Amri MS, Susanti D, Abdul Kudos MB, Md Nor NFA, Syukri Y. 2020. Medicinal uses, phytochemistry, and pharmacological properties of Piper aduncum L. Sains Malaysiana. 49:1829–1851. DOI: https://doi.org/10.17576/jsm-2020-4908-07.
Volpe HXL, Fazolin M, Garcia RB, Magnani RF, Barbosa JC, de Miranda MP. 2016. Efficacy of essential oil of Piper aduncum against nymphs and adults of Diaphorina citri. Pest Management Science. 72:1242–1249. DOI: https://doi.org/10.1002/PS.4143.
Tarwotjo U. 2012. Pengaruh ekstrak daun dan ranting Aglaia odorata terhadap parasitasi dan enkapsulasi Eriborus argenteopilosus pada inangnya, Crocidolomia binotalis. Jurnal Bioma: Berkala Ilmiah Biologi. 11:64-68. DOI: https://doi.org/10.14710/bioma.11.2.64-68.
Wahyuni D, Loren I. 2015. Perbedaan toksisitas ekstrak daun sirih (Piper betle L.) dengan ekstrak biji srikaya (Annona squamosa L.) terhadap larva nyamuk Aedes aegypti L. Jurnal Saintifika. 17:38–48.
Wang R, Fang Y, Zhang J, Wang J, Feng H, Luo C. 2021. Characterization of field‐evolved resistance to pyridalyl in a near‐isogenic line of diamondback moth, Plutella xylostella. Pest Management Science. 10:1–27.DOI: https://doi.org/10.1002/ps.6129.
Yodsaoue O, Sonprasit J, Karalai C, Ponglimanont C, Tewtrakul S, Chantrapromma S. 2012. Diterpenoids and triterpenoids with potential anti-inflammatory activity from the leaves of Aglaia odorata. Phytochemistry. 76:83–91. DOI: https://doi.org/10.1016/j.phytochem.2012.01.015.
Yang Sihai, Zeng Shuiyun, Zheng Lieshen. 2004. Insecticidal active constituents from twig of Aglaia odorata. Journal Chinese Traditional and Herbal Drugs. 11: 1207–1211.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Maria Heviyanti, Dadang, Dewi Sartiami, Yayi Munara Kusumah, Purwantiningsih
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
